Lysosomal proton pump activity: supravital cell staining with acridine orange differentiates leukocyte subpopulations.

Acridine orange as a fluorescent probe for lysosomal proton pump.

Acridine orange was found to accumulate in pure lysosomal particles (tritosomes) in vitro, and the quenching of its fluorescence correlated well with the delta pH (inside acid) across the lysosomal membrane. Use of this dye showed that Mg-ATP caused lysosomal acidification. This acidification was sensitive to N,N'-dicyclohexylcarbodiimide, N-ethylmaleimide, and azide, but not to oligomycin, oua...

Euchrysine, a supravital fluorescent lysosomal stain: technic and application for hematologic investigation.

Aloe-Emodin Influence on the Lysosomal Compartment of Hela Cells

Background: Aloe-emodin belongs to the group of anthraquinones having extremely high biological activity. The aim of this study was to evaluate the range of morphological and biochemical changes in HeLa cells treated with aloe-emodin, especially with regard to the lysosomal compartment. Methods: Marking of lysosomes was performed with neutral red staining for conventional light microscopy and a...

Prelysosomal acidic vacuoles in Dictyostelium discoideum

We have examined the ameba Dictyostelium discoideum for evidence of a discrete, prelysosomal, acidic receiving compartment in endocytosis. We observed in the cytoplasm abundant round vacuoles with diameters up to 2 microns that concentrated acridine orange by a process inhibited by 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl). They were therefore taken to be acidic. The vacuoles were observ...

Supravital blood studies, using acridine orange fluorescence.

LTHOUGH FLUORESCENCE microscopy had its beginnings over half a century ago, it has only recently gained widespread usage as a research tool, and practical applications have yet to be fully exploited. Blood has been one of the tissues most extensively studied by fluorochroming,’ but few reports have been published in English. In addition, the previously reported saline dilution technics for supr...